hrp conjugated mouse anti human lambda  (SouthernBiotech)

Journal: Immunity

Article Title: Adeno-associated virus delivery of anti-HIV monoclonal antibodies can drive long-term virologic suppression

doi: 10.1016/j.immuni.2019.02.005

Figure Lengend Snippet: (A) Twelve rhesus macaques were experimentally infected with SHIV-AD8 at week 0 and viral loads were quantitated in plasma over time. At week 36 post-SHIV infection the monkeys were separated into two groups and received intramuscular inoculations of rAAV8 vectors coding for different anti-HIV broadly neutralizing antibodies (the “bi-group”, on the left panel, received 3BNC117 and 10–1074; the “quad-group”, on the right panel, received PGT145, 35O22, N6 and PGT128). At week 60 the monkeys received a booster inoculation of rAAV1 vectors coding for the same corresponding antibodies. Note: the viral load limit of detection (15 copies of viral RNA per ml of plasma) is indicated by a dashed line. Samples below the limit of detection were assigned the values of 5, 7 or 10 for graphing purposes and to avoid overlap. Macaque r13029 had to be euthanized at week 76 post-infection due to SHIV related complications. (B) Extent of virological control in monkeys r14097 and r14121 from (A). (C) Levels of anti-HIV broadly neutralizing antibodies PGT145, 35O22, N6 and PGT128 in serum of monkeys r14097 and r14121 were quantified by ELISA. See also Fig. S3 and Fig. S4.

Article Snippet: In the twelve-monkey study: the AAV-delivered mAb 3BNC117 was captured with the anti-idiotype antibody “1F1” (Celldex Therapeutics); the untagged 10–1074 mAb was captured with an anti-idiotype antibody provided by Celldex Therapeutics (“3A1”); the mAbs 35O22 and PGT145 were quantitated using the anti-C9 antibody “1D4” (Millipore), an anti-human kappa chain (PGT145) or an anti-human lambda chain (35O22) secondary HRP-conjugated antibody was used for detection (both Southern Biotech); Strep-Tactin coated plates (IBA Lifesciences) were used to capture PGT128 and N6, an anti-human kappa chain (N6) or an anti-human lambda chain (PGT128) secondary HRP-conjugated antibody was used for detection (both Southern Biotech).

Techniques: Infection, Enzyme-linked Immunosorbent Assay